10.6 Patch recording and optical imaging

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From the course by Peking University

Advanced Neurobiology I

221 оценки

Peking University

Advanced Neurobiology I

221 оценки

Hello everyone! Welcome to advanced neurobiology! Neuroscience is a wonderful branch of science on how our brain perceives the external world, how our brain thinks, how our brain responds to the outside of the world, and how during disease or aging the neuronal connections deteriorate. We’re trying to understand the molecular, cellular nature and the circuitry arrangement of how nervous system works. Through this course, you'll have a comprehensive understanding of basic neuroanatomy, electral signal transduction, movement and several diseases in the nervous system. This advanced neurobiology course is composed of 2 parts (Advanced neurobiology I and Advanced neurobiology II, and the latter will be online later). They are related to each other on the content but separate on scoring and certification, so you can choose either or both. It’s recommended that you take them sequentially and it’s great if you’ve already acquired a basic understanding of biology. Thank you for joining us!

From the lesson

Techniques in neuroscience

Let's learn some prevalent techniques in neuroscience.

10.1 Observation and perturbation10:53

10.2 Animal models in neurological research17:02

10.3 Genetic and molecular techniques12:33

10.4 Anatomical techniques15:37

10.5 Mapping synaptic connections6:48

10.6 Patch recording and optical imaging3:27

Meet the Instructors

Yan Zhang
Professor
School of Life Sciences, Peking University
Yulong Li
Research Fellow
School of Life Sciences, Peking University

And there are many other different ways as I mentioned, for

example, to control a neuronal activity,

to recall a neuronal activity with electrophysiology, and for

example, using many, many electrodes at the same time.

Imagine how that put into a monkey's brain.

And then you can recall many neurons, and you can recall single cells, and

then you can put into a natural in a single cell.

For example, if your skillset are very, very good, you can put one electrode.

Putting a blue color here, you can see this blue color through this cell.

You put in another electrode in a very teeny,

tiny dendrites and then filter another cell.

So you can simultaneously recall a single cell with two electrodes,

one in the soma and one in the dendrite.

In the world, probably only 20 labs can do that.

And then of course, one can recall a single cells and

single channel from those cells.

And then more importantly, recent development of this genetic technique and

the imaging technique together to generate this imaging sensor allow much,

much more people can access the neuronal activity by imaging.

Because imaging are much more easier than putting electrode to recall a cell.

And then this illustrates that a calcium imaging can image the action

potential because here is electrical recording from the cell.

You can see whenever the cell action potential,

that's a constant signal that corresponding to the electrical firing.

Then from this calcium imaging,

you can indirectly get in the information of these neurons.

And then you can perturb those neuronal activity by

channel reduction, by direct electrical stimulation.

So we can go on and on.

And with that, I'm suggesting that people should take

a look of this chapter by mode.

And then we're going to have a more better overview of the neural techniques.

Again, it's new ways to observe and

perturb the neuronal activity, and one needs to really,

sort of understand what is the challenger in the complicated anatomy.

Faster reaction of the brain to design and

invent new techniques to trace their connections and

then to understand and perturb the neuronal activities.

All right, with that let's just call it an end for today,

and I hope you guys will enjoy the rest of Nang Lee sections.

I can see so many of you are so happy, I can understand that.

And then I wish at this with my sections,

I secretly hope some of you like or

love learning neuroscience a little bit more.

I hope I don't scare you guys away.

And I hope one day all of you will have the new findings or

new techniques in different aspect of

neuroscience that can allow me to be proud of.

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